Genetic diversity of Botritis cinerea revealed by sequencing and identification of B. otrytis cinerea isolates in strawberries of Almaty region
Main Article Content
A.Zh. Zhaxylykov
S.Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.
alikhanzhaxylykov33@gmail.com
A.B Ismagulova
S.Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
altynai14.02@mail.ruAbstract
This article discusses the isolation and identification of 8 isolates of strawberry gray mold pathogens from four varieties in
the Almaty region: Vivara, Murano, Serofline and Albion. The morpho-cultural characteristics of the isolated Botrytis cinerea
(B. cinerea) strains were studied, and the rates of radial colony growth in various nutrient media were determined. Percent
identity of eight Botrytis cinerea isolates ranged from 95.4% to 100%. For most isolates, percent identity exceeded 99%,
indicating a high degree of genetic conservatism in the ITS regions. An exception was isolate K12 (PI = 95.4%), which exhibited
minor nucleotide variations that, however, did not affect the taxonomic classification. The isolated strains were deposited in
the GenBank database maintained by the National Center for Biotechnology Information (NCBI): B. cinerea K1-PV226173.1,
B. cinerea K2-PV226178.1, B. cinerea K9-PV203239.1, B. cinerea K12- PV203241.1, B. cinerea K13-PV203240.1, B. cinerea
K24-PV203243.1, B. cinerea K27-PV203242.1, B. cinerea K28-PV203244.1. The constructed phylogenetic tree shows that
B. cinerea forms a large monophyletic cluster with high intraspecific variability, which confirms its wide distribution. Other
Botrytis species form polyphyletic and stable groups, reflecting their taxonomic isolation.
Article Details
Accepted 2025-03-28
Published 2025-03-31
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