ИДЕНТИФИКАЦИЯ БАКТЕРИАЛЬНЫХ ИЗОЛЯТОВ ИЗ ПОЧВ РЕГИОНА АРАЛЬСКОГО МОРЯ
Основное содержимое статьи
Rabiga Uakhit
LLP «National Center for Biotechnology», Astana, Kazakhstan
erken.uakhitrabiga@gmail.com
Shuga Manabayeva
LLP «National Center for Biotechnology», Astana, Kazakhstan
manabayeva@biocenter.kzАннотация
Регион Аральского моря представляет собой экстремальную засушливо-соленую среду, сформировавшуюся в результате длительного высыхания, засоления и деградации прибрежных и бывших донных почв. Целью данного исследования была молекулярно-генетическая и таксономическая идентификация культивируемых бактериальных изолятов, выделенных из почв региона Аральского моря. Образцы почвы были собраны с 9 участков отбора проб, расположенных в прибрежной зоне, прилегающей к высохшему дну Аральского моря. В общей сложности было получено 42 бактериальных изолята, охарактеризованных по морфологии колоний и микроскопии, и идентифицированных с помощью секвенирования гена 16S рРНК с последующим филогенетическим анализом в MEGA11. Изоляты были отнесены к 23 видам, принадлежащим к 11 родам, включая Priestia, Bacillus, Planomicrobium, Paenibacillus, Streptomyces, Microbacterium, Sphingobium, Sphingomonas, Pseudomonas, Acinetobacter и Acidovorax. Распределение видов было неравномерным, с преобладанием Paenibacillus, Bacillus и Pseudomonas. Среди наиболее часто встречающихся таксонов были Paenibacillus xylanexedens, Paenibacillus barcinonensis, Bacillus subtilis и Pseudomonas putida. Филогенетическая реконструкция, основанная на 24 нуклеотидных последовательностях и 604 выровненных позициях, подтвердила кластеризацию в соответствии с таксономической принадлежностью. Полученные результаты демонстрируют существенную таксономическую гетерогенность культивируемых бактерий в почвах Аральского моря и служат основой для дальнейших исследований их экологической роли и биотехнологического потенциала.
Информация о статье
##plugins.generic.dates.accepted## 2026-05-06
##plugins.generic.dates.published## 2026-06-27
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Literature
Jiang, H., Huang, J., Li, L., Huang, L., Manzoor, M., Yang, J., Wu, G., Sun, X., Wang, B., Egamberdieva, D., Panosyan, H., Birkeland, N. K., Zhu, Z., Li, W. Onshore soil microbes and endophytes respond differently to geochemical and mineralogical changes in the Aral Sea // The Science of the Total Environment. – 2021. – Vol. 765. – P. 142675. https://doi.org/10.1016/j.scitotenv.2020.142675.
Wicaksono, W. A., Egamberdieva, D., Berg, C., Mora, M., Kusstatscher, P., Cernava, T., Berg, G. Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea // mSystems. – 2022. – Vol. 7(6). – P. e00739-22. https://doi.org/10.1128/msystems.00739-22.
Šimonovičová, A., Pauditšová, E., Nosalj, S., Oteuliev, M., Klištincová, N., Maisto, F., Kraková, L., Pavlović, J., Šoltys, K., Pangallo, D. Fungal and Prokaryotic Communities in Soil Samples of the Aral Sea Dry Bottom in Uzbekistan // Soil Systems. – 2024. – Vol. 8(2). – P. 58. https://doi.org/10.3390/soilsystems8020058.
Shurigin, V., Hakobyan, A., Panosyan, H., Egamberdieva, D., Davranov, K., Birkeland, N. K. A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups // MicrobiologyOpen. – 2019. – Vol. 8(9). – P. e00850. https://doi.org/10.1002/mbo3.850.
Черных, Н. А., Меркель, А. Ю., Кондрашева, К. В., Алимов, Ж. Э., Клюкина, А. А., Бонч-Осмоловская, Е. А., Слободкин, А. И., Давранов, К. Д. У берегов исчезающего моря: микробные сообщества Арала и южного Приаралья // Микробиология. – 2024. – Т. 93(1). – С. 3–16. https://doi.org/10.31857/S0026365624010035.
Etesami, H., Beattie, G. A. Mining Halophytes for Plant Growth-Promoting Halotolerant Bacteria to Enhance the Salinity Tolerance of Non-halophytic Crops // Frontiers in Microbiology. – 2018. – Vol. 9. – P. 148. https://doi.org/10.3389/fmicb.2018.00148.
Gao, Y., Zou, H., Wang, B., Yuan, F. Progress and Applications of Plant Growth-Promoting Bacteria in Salt Tolerance of Crops // International Journal of Molecular Sciences. – 2022. – Vol. 23(13). – P. 7036. https://doi.org/10.3390/ijms23137036.
Aytenov, I. S., Bozorov, T. A., Zhang, D., Samadiy, S. A., Muhammadova, D. A., Isokulov, M. Z., Murodova, S. M., Zakirova, O. R., Chinikulov, B. K., Sherimbetov, A. G. Uncovering the Antifungal Potential of Plant-Associated Cultivable Bacteria from the Aral Sea Region against Phytopathogenic Fungi // Pathogens. – 2024. – Vol. 13(7). – P. 585. https://doi.org/10.3390/pathogens13070585.
Gao, L., Ma, J., Liu, Y., Huang, Y., Mohamad, O. A. A., Jiang, H., Egamberdieva, D., Li, W., Li, L. Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin // Microorganisms. – 2021. – Vol. 9(7). – P. 1448. https://doi.org/10.3390/microorganisms9071448.
Liu, W., Ma, L., Abuduwaili, J. Historical Change and Ecological Risk of Potentially Toxic Elements in the Lake Sediments from North Aral Sea, Central Asia // Applied Sciences. – 2020. – Vol. 10(16). – P. 5623. https://doi.org/10.3390/app10165623.
Yaakoub, H., Sanchez, N. S., Ongay-Larios, L., Courdavault, V., Calenda, A., Bouchara, J. P., Coria, R., Papon, N. The high osmolarity glycerol (HOG) pathway in fungi // Critical Reviews in Microbiology. – 2022. – Vol. 48(6). – P. 657–695. https://doi.org/10.1080/1040841X.2021.2011834.
Matzhanova, K. K., Orel, M. M., Matzhanov, T. K. The Aral Sea crisis and its impact on the current state of water bodies and higher aquatic vegetation // ESJ. – 2021. – Vol. 2. – P. 82–86.
Musa, H., Kasim, F. H., Gunny, A. A. N., Gopinath, S. C. Salt-adapted moulds and yeasts: Potentials in industrial and environmental biotechnology // Process Biochemistry. – 2018. – Vol. 69. – P. 33–44. https://doi.org/10.1016/j.procbio.2018.03.026.
Osman, J. R., Fernandes, G. R., Kamilova, E. et al. Genomic microbiome analyses of surface sand samples from the Kyzyl-Kum Desert (Uzbekistan): characterization and comparative study // Archives of Microbiology. – 2023. – Vol. 205. – P. 90. https://doi.org/10.1007/s00203-023-03432-z.
Chauhan, P., Singh, M., Sharma, A., Singh, M., Chadha, P., Kaur, A. Halotolerant and plant growth-promoting endophytic fungus Aspergillus terreus CR7 alleviates salt stress and exhibits genoprotective effect in Vigna radiata // Frontiers in Microbiology. – 2024. – Vol. 15. – P. 1336533. https://doi.org/10.3389/fmicb.2024.1336533.
Li, Y., Li, W., Jiang, L., Li, E., Yang, X., Yang, J. Salinity affects microbial function genes related to nutrient cycling in arid regions // Frontiers in Microbiology. – 2024. – Vol. 15. – P. 1407760. https://doi.org/10.3389/fmicb.2024.1407760.
Austin, P., Mackay, A., Palagushkina, O., Leng, M. A High-Resolution Diatom-Inferred Palaeoconductivity and Lake Level Record of the Aral Sea for the Last 1600 Yr // Quaternary Research. – 2007. – Vol. 3. – P. 383–393. https://doi.org/10.1016/j.yqres.2007.01.009.
Aripov, T., Kukanova, S., Zaynitdinova, L., Tashpulatov, J. Microorganisms of the extreme zones of the Southern Aral Sea region // Scientia. – 2017. – Vol. 20(1). – P. 8–13.
Saitou, N., Nei, M. The neighbor-joining method: A new method for reconstructing phylogenetic trees // Molecular Biology and Evolution. – 1987. – Vol. 4. – P. 406–425.
Tamura, K., Nei, M., Kumar, S. Prospects for inferring very large phylogenies by using the neighbor-joining method // Proceedings of the National Academy of Sciences of the USA. – 2004. – Vol. 101. – P. 11030–11035.
Tamura, K., Stecher, G., Kumar, S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11 // Molecular Biology and Evolution. – 2021. https://doi.org/10.1093/molbev/msab120.
Zhang, G., Bai, J., Zhai, Y., Jia, J., Zhao, Q., Wang, W., Hu, X. Microbial diversity and functions in saline soils: A review from a biogeochemical perspective // Journal of Advanced Research. – 2024. – Vol. 59. – P. 129–140. https://doi.org/10.1016/j.jare.2023.06.015.
Gamalero, E., Bona, E., Todeschini, V., Lingua, G. Saline and Arid Soils: Impact on Bacteria, Plants, and their Interaction // Biology. – 2020. – Vol. 9(6). – P. 116. https://doi.org/10.3390/biology9060116.
Bhatt, H. B., Singh, S. P. Diversity of Cultivable Bacteria in a Saline Desert of Little Rann of Kutch, India: A Phylogenetic Perspective // Frontiers in Marine Science. – 2022. – Vol. 9. – P. 769043. https://doi.org/10.3389/fmars.2022.769043.
Sharma, A., Dev, K., Sourirajan, A., Choudhary, M. Isolation and characterization of salt-tolerant bacteria with plant growth-promoting activities from saline agricultural fields of Haryana, India // Journal of Genetic Engineering and Biotechnology. – 2021. – Vol. 19(1). – P. 99. https://doi.org/10.1186/s43141-021-00186-3.
Hou, Y., Zeng, W., Ao, C., Luo, Y., Wang, Z., Hou, M., Huang, J. Bacillus atrophaeus WZYH01 and Planococcus soli WZYH02 Improve Salt Tolerance of Maize (Zea mays L.) in Saline Soil // Frontiers in Plant Science. – 2022. – Vol. 13. – P. 891372. https://doi.org/10.3389/fpls.2022.891372.
Tariq, H., Subramanian, S., Geitmann, A., Smith, D. L. Bacillus and Paenibacillus as plant growth-promoting bacteria in soybean and cannabis // Frontiers in Plant Science. – 2025. – Vol. 16. – P. 1529859. https://doi.org/10.3389/fpls.2025.1529859.
Dobrzyński, J., Naziębło, A., Kulkova, I., Szpytma, M., Antosik, A., Sitarek-Andrzejczyk, M., Wróbel, B. Paenibacillus-Pseudomonas Consortium Improves Barley Performance with Minimal Impact on Native Rhizobacterial Community // Microorganisms. – 2026. – Vol. 14(2). – P. 488. https://doi.org/10.3390/microorganisms14020488.
Fan, M., Tan, S., Wang, W., Zhang, X. Improvement in Salt Tolerance Ability of Pseudomonas putida KT2440 // Biology. – 2024. – Vol. 13(6). – P. 404. https://doi.org/10.3390/biology13060404.
Marasco, R., Mosqueira, M. J., Seferji, K. A., Al Romaih, S. M., Michoud, G., Xu, J. et al. Desert-adapted plant growth-promoting pseudomonads modulate plant auxin homeostasis and mitigate salinity stress // Microbial Biotechnology. – 2024. – Vol. 17. – P. e70043. https://doi.org/10.1111/1751-7915.70043.
Gao, Y., Han, Y., Li, X., Li, M., Wang, C., Li, Z., Wang, Y., Wang, W. A Salt-Tolerant Streptomyces paradoxus D2-8 from Rhizosphere Soil of Phragmites communis Augments Soybean Tolerance to Soda Saline-Alkali Stress // Polish Journal of Microbiology. – 2022. – Vol. 71(1). – P. 43–53. https://doi.org/10.33073/pjm-2022-006.
Nozari, R. M., Ramos, L. M., da Luz, L. A., Almeida, R. N., Lucas, A. M., Cassel, E. et al. Halotolerant Streptomyces spp. induce salt tolerance in maize through systemic induction of the antioxidant system and accumulation of proline // Rhizosphere. – 2022. – Vol. 24. – P. 100623. https://doi.org/10.1016/j.rhisph.2022.100623.
Boss, B. L., Wanees, A. E., Zaslow, S. J., Normile, T. G., Izquierdo, J. A. Comparative genomics of the plant-growth promoting bacterium Sphingobium sp. strain AEW4 isolated from the rhizosphere of the beachgrass Ammophila breviligulata // BMC Genomics. – 2022. – Vol. 23(1). – P. 508. https://doi.org/10.1186/s12864-022-08738-8.
Wang, F., Wei, Y., Yan, T., Wang, C., Chao, Y., Jia, M., An, L., Sheng, H. Sphingomonas sp. Hbc-6 alters physiological metabolism and recruits beneficial rhizosphere bacteria to improve plant growth and drought tolerance // Frontiers in Plant Science. – 2022. – Vol. 13. – P. 1002772. https://doi.org/10.3389/fpls.2022.1002772.
Gupta, R. S., Patel, S., Saini, N., Chen, S. Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species // International Journal of Systematic and Evolutionary Microbiology. – 2020. – Vol. 70(11). – P. 5753–5798. https://doi.org/10.1099/ijsem.0.004475.
Youseif, S. H., Abd El-Megeed, F. H., Humm, E. A., Maymon, M., Mohamed, A. H., Saleh, S. A., Hirsch, A. M. Comparative Analysis of the Cultured and Total Bacterial Community in the Wheat Rhizosphere Microbiome Using Culture-Dependent and Culture-Independent Approaches // Microbiology Spectrum. – 2021. – Vol. 9(2). – P. e00678-21. https://doi.org/10.1128/Spectrum.00678-21.
Khan, N., Humm, E. A., Jayakarunakaran, A., Hirsch, A. M. Reviewing and renewing the use of beneficial root and soil bacteria for plant growth and sustainability in nutrient-poor, arid soils // Frontiers in Plant Science. – 2023. – Vol. 14. – P. 1147535. https://doi.org/10.3389/fpls.2023.1147535.
Kumar, A., Singh, S., Gaurav, A. K., Srivastava, S., Verma, J. P. Plant Growth-Promoting Bacteria: Biological Tools for the Mitigation of Salinity Stress in Plants // Frontiers in Microbiology. – 2020. – Vol. 11. – P. 1216. https://doi.org/10.3389/fmicb.2020.01216